What is IVF (In Vitro Fertilization) OR Test Tube Baby ?
In vitro fertilization (IVF) or Test Tube Baby is a fertility treatment in which sperm and eggs are combined in a laboratory. The resulting embryo or embryos are transferred to the woman’s uterus, where they grow into a baby. The treatment can be performed using your own eggs and sperm, or using either donated sperm or donated eggs, or both.
IVF or Test Tube Baby can be used to treat infertility with the following patients :
- Blocked or damaged fallopian tubes
- Malefactor infertility
- including decreased sperm count or sperm motility
- Women with ovulation disorders, premature ovarian failure, uterine fibroids
- Women who have had their fallopian tubes removed
- Individuals with a genetic disorder
- Unexplained infertility
There are five basic steps in the IVF or Test Tube Baby and embryo transfer Procedure:
Monitor and stimulate the development of healthy eggs in the ovaries.
Collect the eggs.
Secure the sperm.
Combine the eggs and sperm together in the laboratory and provide the appropriate environment for fertilization and early embryo growth.
Transfer embryos into the uterus.
A state-of-the-art destination for infertility management, uterus preserving treatment, ensuring safe motherhood. The emphasis is on providing cost-effective solutions with a focus on minimally invasive technology.
The success rate depends on a number of factors including reproductive history, maternal age, cause of infertility, and lifestyle factors. It is also important to understand that pregnancy rates are not the same as live birth rates.
In the India, the live birth rate for each IVF cycle started is approximately:
Pearl Womens Hospital and Yash IVF have 1000+ successful IVF treatment or Test Tube Baby cases having best success rate.
Fertility Management at Pearl Womens Hospital
A recent survey found 46 per cent of couples in the age group 31-40 years to be infertile in India.The problem may lie with either or both the partners. Some of the factors affecting fertility for males include low sperm count, poor motility and diabetes, while for women they could be rated as polycystic ovarian disease, tubal factor, anovulation, endometriosis and endocrine problems.
At Pearl Womens Hospital, we believe “Infertility” is a misnomer.And with this strong conviction and faith, we manage your “Sub fertility”. All through your fertility journey, our team assists you and provides you with treatment options which increase your chances of conception. The so called “Sub-fertility” causes enormous physical and emotional stress to the couple impacting the couple’s health and social well being. The financial cost of treatment of sub-fertility may also prove to be burdensome.
We have made it a mission to give the gift of parenthood to families at an affordable cost.
Artificial Insemination-“Infertility Treatment for men who have low sperm counts or sperm ?”
Artificial insemination is a technique that can help treat certain kinds of infertility in both men and women. It’s a popular
infertility treatment for men who have low sperm counts or sperm
that aren’t strong enough to swim through the cervix and up into the fallopian tubes. Artificial insemination is also sometimes an option for women who have endometriosis or abnormalities of any of their reproductive organs.
Ideally, it makes pregnancy possible where it wasn’t before. Intrauterine insemination (IUI), in which the sperm is placed in the uterus, is the most common form of artificial insemination.
Though the pregnancy rates for women undergoing artificial insemination may not be as high as they are for some more advanced techniques, this technique has a key advantage: It’s a simple procedure with few side effects. For those reasons, we may recommend it as an initial form of treatment for infertility.
IVF – (In Vitro Fertilization)
IVF is never the first step in the treatment of infertility.
IVF involves combining eggs and sperm outside the body in a laboratory. Once an embryo or embryos form, they are then placed in the uterus.
IVF may be an option if you or your partner have been diagnosed with:- • Problems with the fallopian tubes • Endometriosis • Problems with ovulation • Antibody problems that harm sperm or eggs • The inability of sperm to penetrate or survive in the cervical mucus • An unexplained fertility problem
Instead, it’s reserved for cases in which other methods such as fertility drugs, surgery, and artificial insemination haven’t worked.
Intracytoplasmic sperm injection(ICSI)
ICSI is an assisted reproductive technology (ART) used to treat sperm-related infertility problems for couples when little or no sperm are ejaculated in the semen. It is used to enhance the fertilization phase of in vitro fertilization (IVF)by injecting a single sperm into a mature egg. The fertilized egg is then placed in a woman’s uterus or fallopian tube.
At Pearl, our doctors may advise ICSI to couples with very low sperm count and/or low sperm motility even after repeated In Vitro fertilization has been unsuccessful.
Intra-Cytoplasmic Morphologically Selected Sperm Injection(IMSI)
IMSI is the most current and latest technique being used in the treatment for infertility.
A microscope is used in IMSI which magnifies the image of the sperm 7,200 times, thereby allowing doctors to pick the best looking healthier sperms. This results in better egg fertilization rates, better quality embryos, better rate of blastocyst formation and therefore better pregnancy rates.
IMSI is an advanced version of the earlier technique of ICSI (Intracytoplasmic Sperm Injection) having the magnification capacity of 16 times higher than ICSI.
At Pearl Women’s Hospital, a donor is screened for medical fitness before semen is collected from him.
The semen thus obtained is either used immediately for insemination or is stored at -1960C for six months. After 6 months the donor is retested for any infection, particularly for HIV. If the tests are negative, sample is released from quarantine for insemination.
The success rate of both fresh as well as frozen semen is almost similar. However in the case of stored sample, motility decreases rapidly within 12 hours, in contrast to fresh insemination, where the sperms stay alive in the body for 48-72 hours. The major advantage of frozen samples is its minimal risk of HIV infection.
When sperm is not present in the ejaculate due to obstruction, surgical sperm recovery may be recommended in order to collect sperm for treatment.
In this procedure sperm is directly collected from the testes under local anaesthesia on an OPD basis. Sufficient sperm can be obtained in 80 percent of procedures. Sperm collected in this way can then be used in ICSI.
If possible, we obtain enough sperm for freezing as well. This may mean that you will not need another sperm recovery procedure if future ICSI cycles are undertaken, although fresh sample is always preferred. Men can usually go to work the day after this procedure.
At Pearl Women’s Hospital & Yash IVF, both the donor and the recipient are made to understand the procedure and are given psychological counseling prior to an egg donation treatment cycle. The treatment cycle consists of having the donor go through hormone therapy for ovulation stimulation while the recipient’s uterus is synchronized to prepare for the embryo transfer.
By using IVF technology, our fertility specialist retrieves the eggs from the ovaries of the donor, which are then either fertilized by the sperm of the recipient’s male partner or through a sperm donor. The resulting embryo(s) are then implanted into the recipient’s uterus.
The donor’s contribution is to provide the genetic material, but the pregnancy (gestation), labour and birth, and parenting falls to the recipient. If pregnancy is achieved, the resulting child will be genetically related to the recipient’s partner if his sperm was used, but not to the recipient.
Women who may need egg donation include those with
• Poor egg quality and/or diminished ovarian function due to advanced maternal age or surgery/illness • Unexplained infertility • Congenital absence of eggs or ovaries • Prior cancer treatments • A high risk of transmitting genetic diseases
A couple’s infertility problem may also be due to a combination of physical factors that affect both the man and woman. If standard IVF treatment fails or offers very small chance of success, the transfer of a donor embryo into the woman’s womb may be the only way to conceive.
Recent advances in blastocyst culture and transfer have resulted in improved IVF pregnancy rates and reduced multiple pregnancy rates. Traditionally, embryos are transferred to the uterus on the third day (called Day 3 transfer) after fertilization and it is not uncommon to transfer three or four embryos.
However, it is now possible to grow embryos in the laboratory to the blastocyst stage of development, which occurs on day five after fertilization when the embryo has 50-200 cells. Typically, the strongest, healthiest embryos make it to blastocyst stage as they have survived key growth and division processes and have a better chance of implanting once transferred. The selection of potentially more viable embryos allows the embryologist to transfer fewer embryos, often one or two, lowering the risk of high order multiples while maintaining high pregnancy rates. Blastocyst transfer technique is most successful in women who are younger and have a large number of eggs available at retrieval. On average, women with six or more high quality embryos on Day 3 are the best candidates for blastocyst culture because there is a better chance of more embryos growing successfully to Day 5.
At Pearl Women’s Hospital, we recognise that everyones circumstances are different and hence our fertility doctor will discuss with you whether blastocyst transfer is appropriate in your own situation.
Pearl Women’s Hospital and Yash IVF offers a surrogacy program to everyone; couples as well as single parents desirous of parenthood.
We have a large data bank of surrogates who have been thoroughly screened for any infectious disease as well as any psychological disorders. All the potential surrogates are counselled at the clinic along with their husband/partner and are made to understand the entire process before they are enrolled as potential surrogates.
Our nursing staff regularly makes monitoring visits to the surrogates during the nine months of pregnancy to ensure they are taking healthy diet and medicines regularly. Our surrogacy program thus enjoys a high success rate.
Vitrification is the newest method where in cells are frozen rapidly by using very high concentration of cryoprotectant.
The embryos or oocytes are plunged directly into the liquid nitrogen. The result is a solid glass-like cell, free of ice crystals. Vitrification cools the cells in an extraordinarily faster speed so that there is no time for ice crystals to be formed, but instead a kind of gel appears leaving the cells undamaged.
In the classical freezing method, embryos were slowly cooled in order to avoid ice crystal formation around & within the cells.Vitrification offers improved survival rate of the embryos as well as oocytes as compared to classical slow freezing method. This results in better rates of implantation & pregnancy.
At Pearl Women’s Hospital and Yash IVF, we have adopted this method due to its high success rates.
Assisted Laser Hatching
The laser assisted hatching is an additional lab technique designed to improve the efficiency of assisted reproductive technology procedures such as IVF & ICSI. The unfertilised egg is surrounded by a shell called zona pellucida The zona has an important role in fertilisation as it allows only one sperm to penetrate the zona and enter the egg to achieve fertilisation.
After fertilisation of the egg, during the cleavage stage of an embryo, zona pellucida hardens. This development is normal and the purpose is to keep the cells in the egg together. The embryos have to “hatch” or break out of the zona in order to embed into the endometrium lining of the uterine cavity. This occurs about 2-3 days after embryo transfer when the embryo is at the blastocyst stage. Naturally this takes place by expanding/contracting of the zona until it distorts, allowing the blastocyst to “hatch”.
When sperm and eggs are cultured in IVF laboratory, the zona pellucida hardens at much faster rate than natural, making it more difficult for the embryo to implant. Prior to implantation, the embryo has to escape out of its zona pellucida by a process known as hatching. If this process is not completed properly, implantation failure occurs and a pregnancy cannot be achieved.
Assisted hatching is the process of creating a hole in zona pellucida, to aid the embryo in the hatching process.
At Pearl Women’s Hospital & Yash IVF, we offer the Laser technology for Assisted Hatching (LAH),
where a precision laser beam is focused over the zona pellucida making a small opening, between 10-20 microns to facilitate embryo hatching. LAH is done just before the Embryo Transfer.
This helps to increase pregnancy rates by improving implantation rates, since embryo hatching is facilitated. A fast, safe, simple method compared to the old method of hatching which was performed by using acidic medium.
Our doctors may recommend this to women:
with day 3 embryos having a thick Zona
with failed previous IVF/ICSI cycles.
older than 37 years of age, have a tendency to produce eggs with a harder zona pellucida than younger women.
with a high Follicle Stimulating Hormone (FSH) level.
undergoing IVF/ICSI for the first time, who are considered poor responders because they have required
who in an earlier IVF cycle had low fertilisation rate, resulting in three or fewer embryos.
who request laser assisted hatching and are fully informed of its use and benefits.
Contact Pearl Women’s Hospital for more information about IVF treatment Cost.